Thermo Fisher Scientific ERK1 Polyclonal Antibody
ERK1 단백질을 검출하기 위한 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. WB, IHC, ICC, Flow 등 다양한 응용 가능. 1 mg/mL 농도의 액상 형태로 제공되며, 항원 친화 크로마토그래피로 정제됨. MAPK3 신호전달 연구에 적합.
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Thermo Fisher Scientific ERK1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Chicken, Fruit fly, Human, Mouse, Sheep, Rat, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 35 residue synthetic peptide corresponding to rat Erk1 MAP kinase with CGG spacer group added and coupled to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735769 |
Product Specific Information
A 1:1,000 dilution of PA5-77818 was sufficient for detection of ERK1/2 in 20 µg of HeLa cell lysate by ECL immunoblot analysis. Detects approximately 44 kDa (ERK1) and approximately 42 kDa (ERK2).
Target Information
MAPK3 (ERK1) is a serine/threonine kinase that activates p90, RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. ERK1 regulates meiosis, mitosis, and post-mitotic functions in differentiated cells. It is activated by various stimuli including growth factors, cytokines, viral infection, and GPCR ligands. ERK1 translocates to the nucleus upon activation and phosphorylates nuclear targets, influencing proliferation, differentiation, and cell cycle progression. Alternative splicing produces multiple isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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