Thermo Fisher Scientific Crystallin Alpha B Monoclonal Antibody (CRYAB/4661)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: Assay-dependent

Immunohistochemistry (PFA fixed) (IHC (PFA))

• Tested Dilution: 1–2 µg/mL

Peptide Array (Array)

• Tested Dilution: Assay-dependent

Product Specifications

Target Information

Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found in vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens.

The mammalian lens contains three major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of two primary gene products, alpha-A and alpha-B. There are at least five different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, with at least five gamma crystallins identified in bovine and rat lens.

Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone-like manner by preventing the formation of aggregates possibly leading to cataract formation. Phosphorylated states of alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander’s disease, where it accumulates in brain cells of those afflicted.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.