Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), eBioscience
인간 CD107a(LAMP-1)를 인식하는 eBioH4A3 단클론 항체로, Flow Cytometry, IHC, ICC, WB에 적합합니다. 고순도(>90%)로 정제되었으며, 0.5 mg/mL 농도의 액상 형태입니다. 세포 탈과립 분석 및 표면 발현 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5 µg/test | 2 |
| Immunohistochemistry (IHC) | - | 1 |
| Immunohistochemistry (Paraffin) (IHC-P) | 5 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | 16 |
| Flow Cytometry (Flow) | 0.5 µg/test | 18 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Cynomolgus monkey, Human, Non-human primate |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioH4A3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2864970 |
Available Conjugated Formats
- Alexa Fluor 488
- Brilliant Violet 421
- Brilliant Violet 650
- Brilliant Violet 711
- eFluor 450
- eFluor 660
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Custom conjugation available upon request
Product Specific Information
The eBioH4A3 monoclonal antibody reacts with human CD107a (LAMP-1), a 110 kDa glycoprotein predominantly localized in lysosomal/endosomal membranes. CD107a transiently appears on the surface of degranulating cytolytic T cells and is upregulated on activated platelets and certain cancer cells.
Applications Reported:
Suitable for flow cytometry, immunohistochemistry, immunocytochemistry, and western blotting. Also applicable for surface staining in degranulation assays.
Applications Tested:
Validated for intracellular staining, flow cytometry, ICC, IHC (formalin-fixed paraffin embedded tissue, high/low pH antigen retrieval), and WB.
- Flow cytometry: ≤0.5 µg/test (100 µL volume, 10⁵–10⁸ cells/test)
- ICC/IHC: ≤5 µg/mL
Antibody titration is recommended for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
LAMP1 (CD107a) is a major lysosomal membrane protein, also found (1–2%) on the plasma membrane. It contains a signal peptide, 18 N-linked glycosylation sites, a transmembrane domain, and a short cytosolic tail. LAMP1 is essential for lysosome biogenesis and fusion with phagosomes.
Cell surface LAMP1 and LAMP2 promote adhesion of PBMCs to vascular endothelium, potentially contributing to inflammation. Increased LAMP1 expression has been observed in neurons and glial cells near senile plaques in Alzheimer’s disease, as well as in thymic epithelial cells and macrophages during acute thymic involution.
LAMP1 serves as a reliable marker for mast cell activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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