Thermo Fisher Scientific Human IL-21 Recombinant Protein
인간 IL-21 재조합 단백질로 면역세포 활성 연구에 적합합니다. E. coli 발현, >95% 순도, 낮은 엔도톡신 수준(<0.1 ng/µg). IFN-γ 분비를 유도하는 생물학적 활성 확인됨. ELISA, WB, IHC 등 다양한 기능적 분석에 활용 가능.
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- Protein
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Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | QDRHMIRMRQ LIDIVDQLKN YVNDLVPEFL PAPEDVETNC EWSAFSCFQK AQLKSANTGN NERIINVSIK KLKRKPPSTN AGRRQKHRLT CPSCDSYEKK PPKEFLERFK SLLQKMIHQH LSSRTHGSED S |
| Molecular Weight | 15 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >95% by SDS-PAGE |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 = 1–5 ng/mL; determined by IFN-γ secretion from human NK-92 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice (domestic); Wet or Dry ice (international) |
Product Specific Information
Carrier-Free
- Reconstitution: Briefly centrifuge vial before opening. Reconstitute lyophilized recombinant human IL-21 in sterile distilled water to 0.1–1.0 mg/mL. Further dilutions should be made in low endotoxin medium or buffered solution containing a carrier protein (e.g., heat-inactivated FCS or tissue culture grade BSA).
- Storage: Store lyophilized recombinant human IL-21 at 2–8°C, preferably desiccated. After reconstitution, aliquot and store at ≤–20°C. Avoid repeated freeze/thaw cycles.
Target Information
IL-21 is a 17 kDa immunomodulatory cytokine mainly produced by NKT, Th17, and TFH cells. In TFH cells, IL-21 signaling through IL-21R and STAT3 activates Bcl6, essential for TFH cell development and function. IL-21 promotes T cell-dependent B cell differentiation into plasma and memory cells, stimulates IgG production, and induces apoptosis in naive B cells.
In Th17 cells, IL-21 enhances IL-23R expression via STAT3, IRF4, and RORγt, supporting maturation and maintenance by IL-23. It also downregulates Foxp3, influencing immune balance. IL-21 deficiency protects mice from chemically-induced colitis, while elevated IL-21 levels are observed in colitis models.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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