Thermo Fisher Scientific Human CCL13 (MCP-4) Recombinant Protein
인간 CCL13(MCP-4) 재조합 단백질로, 염증 반응 관련 연구에 적합합니다. E. coli 발현 시스템에서 생산되었으며, 98% 이상 순도와 낮은 엔도톡신 수준을 제공합니다. 화학주성 및 면역학적 기능 분석에 활용 가능합니다. Carrier-free 형태로 제공됩니다.
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Thermo Fisher Scientific Human CCL13 (MCP-4) Recombinant Protein
Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | Assay-dependent | |
| Immunohistochemistry (IHC) | Assay-dependent | |
| ELISA | Assay-dependent | |
| Functional Assay | Assay-dependent | |
| Control (Ctrl) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | Human CCL13 recombinant protein contains 75 amino acids |
| Molecular Weight | 8.6 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >98% by SDS-PAGE |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 = 10–100 ng/mL; determined by dose-dependent chemotaxis of human blood cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice (domestic); Wet or Dry ice (international) |
Product Specific Information
Carrier-Free
Reconstitution:
Briefly centrifuge vial before opening to collect contents at the bottom. Reconstitute in sterile, distilled water to 0.1–1.0 mg/mL. Further dilutions should be made in tissue culture grade buffered solutions with heat-inactivated fetal calf serum or low endotoxin BSA.
Storage:
Store lyophilized at ≤+4°C, preferably desiccated. Store reconstituted MCP-4 at -20°C in working aliquots. Avoid repeated freeze-thaw cycles.
Target Information
Monocyte Chemotactic Protein 4 (MCP-4), also known as CCL13, is induced by inflammatory proteins such as IL-1 and TNFα. MCP-4 acts as a ligand for G protein-coupled receptors CCR2, CCR3, and CCR5. It activates signaling in monocytes, T lymphocytes, eosinophils, and basophils, which are associated with allergic responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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