Thermo Fisher Scientific Human CCL22 (MDC) Recombinant Protein
인간 CCL22(MDC) 재조합 단백질로, T세포 화학유인 활성 평가에 사용됩니다. E. coli 발현, >98% 순도, 저엔도톡신(<0.1 ng/µg)으로 정제되었습니다. ELISA, WB, IHC 등 다양한 실험에 활용 가능하며, 장기 보관 시 -20°C 이하에서 안정적입니다.
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Applications
Western Blot (WB)
- Assay-dependent
Immunohistochemistry (IHC)
- Assay-dependent
ELISA
- Assay-dependent
Functional Assay
- Assay-dependent
Control
- Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | Human CCL22 recombinant protein contains 67 amino acids |
| Molecular Weight | 8 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >98% by SDS-PAGE |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 = 10–100 ng/mL; determined by the dose-dependent chemotaxis of human T-cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice (domestic); Wet or Dry ice (international) |
Product Specific Information
Carrier-Free
Reconstitution:
The vial should be centrifuged prior to opening to bring contents to the bottom. The lyophilized powder should be reconstituted with sterile, distilled water to a concentration of 0.1–1.0 mg/mL. Do not vortex. This solution can be stored at 2–8°C for up to 1 week.
For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (e.g., 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage:
The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long-term storage.
Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C.
Avoid repeated freeze/thaw cycles.
Target Information
CCL22 may play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology.
It is chemotactic for monocytes, dendritic cells, and natural killer cells.
CCL22 is a mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes, but has no chemotactic activity for neutrophils, eosinophils, or resting T-lymphocytes.
Binds to CCR4.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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