Thermo Fisher Scientific RANKL (soluble) Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MEKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human sRANK Ligand. Anti-Human sRANK Ligand-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human sRANK Ligand by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sRANK Ligand (500-P133G) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human sRANK Ligand.

Western Blot:
To detect hsRANKL by Western Blot analysis, use at 0.1–0.2 µg/mL.
Used with compatible secondary reagents, detection limit for Recombinant hsRANKL is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Packaging:
500-P133GBT-1MG will be provided as 2 × 500 µg.

Target Information

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family, which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. It acts as a dendritic cell survival factor and regulates T cell-dependent immune responses.
T cell activation induces expression of this gene, leading to increased osteoclastogenesis and bone loss. The protein activates antiapoptotic kinase AKT/PKB via a signaling complex involving SRC kinase and TRAF6, suggesting a role in apoptosis regulation.
Gene disruption in mice results in severe osteopetrosis and lack of osteoclasts, with defects in T and B lymphocyte differentiation and mammary gland development. Two alternatively spliced transcript variants have been identified.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.