Thermo Fisher Scientific RANKL (soluble) Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific RANKL (soluble) Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MEKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human sRANK Ligand. Anti-Human sRANK Ligand-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human sRANK Ligand by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sRANK Ligand (500-P133) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human sRANK Ligand.

Western Blot:
To detect hsRANKL by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hsRANKL is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Package:
500-P133BT-1MG will be provided as 2 × 500 µg.

Target Information

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family, which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. It acts as a dendritic cell survival factor and regulates T cell-dependent immune responses. T cell activation induces expression of this gene, increasing osteoclastogenesis and bone loss. It activates antiapoptotic kinase AKT/PKB through a complex involving SRC kinase and TRAF6, suggesting a role in apoptosis regulation. Gene disruption in mice causes severe osteopetrosis, lack of osteoclasts, and defects in T/B cell differentiation and mammary gland development. Two alternatively spliced transcript variants have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.