Thermo Fisher Scientific Phospho-NFkB p65 (Ser529) Monoclonal Antibody (B33B4WP), PE, eBioscience
PE 형광 표지된 인간 NFkB p65 (Ser529) 인산화형 특이 단일클론 항체. 세포 내 단백질 염색 및 유세포 분석에 최적화. TNFα 자극 세포에서 65 kDa 밴드 검출. Blue/Green/Yellow-Green 레이저 사용 가능. 연구용으로만 사용.
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Thermo Fisher Scientific Phospho-NFkB p65 (Ser529) Monoclonal Antibody (B33B4WP), PE, eBioscience
Applications
- Flow Cytometry (Intracellular staining)
Tested Dilution: 5 µL (0.06 µg)/test
Publications: View 5 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | B33B4WP |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572750 |
Available Formats
- eFluor 450
- eFluor 660
- PE-Cyanine7
- PerCP-eFluor 710
- Request custom conjugation available
Product Specific Information
The B33B4WP monoclonal antibody recognizes human NFκB p65 subunit when serine 529 is phosphorylated.
NFκB is a transcription factor regulating genes involved in proliferation, apoptosis, immunity, and cancer.
Functional NFκB complexes are composed of p65 (RelA), c-Rel, RelB, p50, and p52 subunits.
Activation by TNFα, IL-1β, LPS, camptothecin, or PMA induces phosphorylation and degradation of IκB, allowing NFκB translocation to the nucleus.
Phosphorylation at Ser529 and/or Ser536 positively regulates p65 activity.
This antibody detects a single band at 65 kDa in TNFα-stimulated HeLa cell lysates.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated for use at 5 µL (0.06 µg)/test. Suitable for 10⁵–10⁸ cells/test.
Recommended Protocols:
- Protocol A: Two-step (cytoplasmic)
- Protocol C: Two-step (Fixation/Methanol)
- Protocol B (nuclear proteins): Not suitable
For detailed instructions, refer to Staining Intracellular Antigens for Flow Cytometry Protocol (BestProtocols®).
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Nuclear factor kB p65 (NF-kB p65), encoded by the RELA gene on chromosome 11, is also known as RelA.
It forms heterodimers with p50 or p52 to regulate transcriptional activity.
TNFα binding induces IKK-mediated phosphorylation of IκB, leading to IκB degradation and NFκB activation.
NFκB p65 plays a key role in transcription of immunoglobulin kappa gene in mature B-lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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