Thermo Fisher Scientific BATF Monoclonal Antibody (MBM7C7), PE, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular Staining)
• Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Available Formats

• eFluor 660
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The MBM7C7 monoclonal antibody reacts with human and mouse BATF (B cell activating transcription factor). BATF is a 14 kDa member of the AP-1 family of transcription factors involved in the repression of other AP-1 family members. It is upregulated in activated T cells and is critical for Th17 differentiation, interacting with promoters of IL17, IL17F, IL21, and IL22. BATF is also implicated in CD8 T cell exhaustion in HIV infection.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated and validated for intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00).
Refer to BestProtocols® for staining protocol (Protocol B: One-step protocol for intracellular (nuclear) proteins).
Recommended use: 5 µL (0.25 µg) per test, in 100 µL final volume. Cell number: 10⁵–10⁸ cells/test (empirical determination required).

Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

BATF is a nuclear basic leucine zipper protein in the AP-1/ATF superfamily. Its leucine zipper mediates dimerization with Jun family members. BATF acts as a negative regulator of AP-1/ATF transcriptional events and inhibits Ras/Fos-mediated transformation. It is essential for differentiation of IL-17-producing T helper (Th17) cells and for proper class-switch recombination in T and B cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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