Thermo Fisher Scientific Synaptophysin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

-

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

-

Immunocytochemistry (ICC/IF)

Assay-dependent

View 4 publications 4 publications

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Sheep, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues G(253) P G G Y G P Q D S Y G P Q G G Y Q P D(272) of rat Synaptophysin. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Synaptophysin,uniProtId:P07825-1,ncbiNodeId:10114,antigenRange:254-272,antigenLength:307,antigenImageFileName:PA1-1043_Synaptophysin_P07825-1_Rabbit.svg,antigenImageFileNamePDP:PA1-1043_Synaptophysin_P07825-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2199026

Product Specific Information

PA1-1043 detects Synaptophysin in rat, mouse, bovine, and sheep samples.

PA1-1043 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a ~33.8 kDa protein representing Synaptophysin in rat, mouse and bovine brain tissue as well as sheep retinas.

The PA1-1043 immunogen is a synthetic peptide corresponding to residues G(253) P G G Y G P Q D S Y G P Q G G Y Q P D(272) of rat Synaptophysin. This sequence is conserved in many species including human, mouse and bovine. The PA1-1043 immunizing peptide (Cat. # PEP-279) is available for use in neutralization and control experiments.

Target Information

Synaptophysin is a calcium-binding and integral membrane glycoprotein present in presynaptic vesicles in almost all neurons. Synaptophysin has four transmembrane domains and it forms a complex with dynamin at high calcium concentrations suggesting an involvement in synaptic vesicle endocytosis. It is also involved in the regulation of short-term and long-term synaptic plasticity. Synaptophysin is currently the most widely used marker for nerve terminals and for differentiating neuroendocrine tumors. Mutations in the gene can result in mental retardation, X-linked 96.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.