Thermo Fisher Scientific S1P1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

2-4 µg/mL

View 8 publications 8 publications

Immunohistochemistry (IHC)

Assay-dependent

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues S(359) H P Q K D D G D N P E T I(372) of mouse S1P1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:S1P1,uniProtId:O08530-1,ncbiNodeId:10090,antigenRange:360-372,antigenLength:382,antigenImageFileName:PA1-1040_S1P1_O08530-1_Rabbit.svg,antigenImageFileNamePDP:PA1-1040_S1P1_O08530-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2184729

Product Specific Information

PA1-1040 detects S1P1 protein in transfected human and mouse cell samples. This antibody shows no cross-reactivity to S1P3.

PA1-1040 has successfully been used in immuno-fluorescence, immunohistochemistry, immunoprecipitation and Western blot procedures. By Western blot, this antibody detects an ~44 kDa protein representing recombinant S1P1 from transfected HEK293 cells. Immunofluorescence data demonstrates that S1P1 is localized to the plasma membrane after staining with PA1-1040.

The PA1-1040 immunogen is a synthetic peptide corresponding to residues S(359) H P Q K D D G D N P E T I(372) of mouse S1P1. This sequence is completely conserved in rat and 92% conserved in human S1P1. This peptide (Cat. # PEP-220) is available for use in neutralization and control experiments.

Target Information

S1P1 is a G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that is coupled to the G(i) subclass of heteromeric G proteins. S1P1 signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. It plays an important role in cell migration via its role in the reorganization of the actin cytoskeleton and formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. S1P1 is required for normal chemotaxis toward sphingosine 1-phosphate, normal embryonic heart development, and normal cardiac morphogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.