Thermo Fisher Scientific GFAP Monoclonal Antibody (2.2B10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

10-50 µg/mL

View 198 publications 198 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 6 publications 6 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 22 publications 22 publications

Immunohistochemistry - Free Floating (IHC (Free))

-

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 45 publications 45 publications

Flow Cytometry (Flow)

Assay-dependent

View 4 publications 4 publications

ELISA (ELISA)

0.1-0.5 µg/mL

-

Immunoprecipitation (IP)

2-5 µg

View 1 publication 1 publication

Inhibition Assays (IA)

-

View 1 publication 1 publication

In vitro Assay (IV)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 9 publications 9 publications

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Cat, Cynomolgus monkey, Ferret, Guinea pig, Human, Mouse, Pig, Rat, Rhesus monkey

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

2.2B10

Immunogen

Enriched bovine glial filaments. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GFAP,uniProtId:Q28115-1,ncbiNodeId:9913,antigenRange:1-428,antigenLength:428,antigenImageFileName:13-0300_GFAP_Q28115-1_Rat.svg,antigenImageFileNamePDP:13-0300_GFAP_Q28115-1_Rat_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2532994

Target Information

GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.