Thermo Fisher Scientific NEFH Monoclonal Antibody (RMdO-20)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

1:20

-

Immunocytochemistry (ICC/IF)

1:20

View 5 publications 5 publications

ELISA (ELISA)

0.1-0.5 µg/mL

-

Immunoprecipitation (IP)

2-5 µg

-

Product Specifications

Species Reactivity

Bovine, Hamster, Human, Mollusc, Mouse, Rabbit, Rat

Published species

Human, Mouse, Rabbit, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

RMdO-20

Immunogen

Adult Rat neurofilaments if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NF-H,uniProtId:P16884-1,ncbiNodeId:10114,antigenRange:1-1072,antigenLength:1072,antigenImageFileName:13-1300_NF-H_P16884-1_House_mouse.svg,antigenImageFileNamePDP:13-1300_NF-H_P16884-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2532999

Product Specific Information

This antibody reacts with 200 kDa subunit protein of human neurofilament. It specifically recognizes a nonphosphorylated epitope in the tail domain of NF-H. Dephosphorylation of NF-H will increase immunoreactivity with this antibody.

This antibody is suitable for immunohistochemical staining of Bouin`s and alcohol-fixed paraffin-embedded or frozen tissue sections. To stain, incubate 30-60 minutes at room temperature or overnight at 4°C.

Target Information

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-H has an important role in mature axons and is involved in amyotrophic lateral sclerosis (ALS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.