Thermo Fisher Scientific NEFH Monoclonal Antibody (RMdO-20)
Neurofilament heavy chain(NF-H)을 인식하는 mouse monoclonal antibody로, 비인산화 epitope에 특이적입니다. Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합하며, 인간 및 설치류 시료에서 반응성이 확인되었습니다. 연구용으로만 사용됩니다.
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Applications 및 Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–2 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | - | View 7 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20 | View 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:20 | - |
| Immunocytochemistry (ICC/IF) | 1:20 | View 5 publications |
| ELISA | 0.1–0.5 µg/mL | - |
| Immunoprecipitation (IP) | 2–5 µg | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Hamster, Human, Mollusc, Mouse, Rabbit, Rat |
| Published Species | Human, Mouse, Rabbit, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RMdO-20 |
| Immunogen | Adult Rat neurofilaments |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2532999 |
Product Specific Information
This antibody reacts with the 200 kDa subunit protein of human neurofilament. It specifically recognizes a nonphosphorylated epitope in the tail domain of NF-H. Dephosphorylation of NF-H increases immunoreactivity with this antibody.
It is suitable for immunohistochemical staining of Bouin’s and alcohol-fixed paraffin-embedded or frozen tissue sections. Incubate for 30–60 minutes at room temperature or overnight at 4°C.
Target Information
Neurofilaments are intermediate filament proteins found specifically in neurons, composed predominantly of NF-L, NF-M, and NF-H. NF-H plays an important role in mature axons and is implicated in amyotrophic lateral sclerosis (ALS). Expression of neuronal intermediate filament proteins is tissue-specific and developmentally regulated.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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