Thermo Fisher Scientific E-cadherin Monoclonal Antibody (HECD-1)

Applications

Tested Dilution

Publications

Western Blot (WB)

2-10 µg/mL

View 44 publications 44 publications

Immunohistochemistry (IHC)

-

View 35 publications 35 publications

Immunohistochemistry (Paraffin) (IHC (P))

2-10 µg/mL

View 46 publications 46 publications

Immunohistochemistry (Frozen) (IHC (F))

2-10 µg/mL

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1 µg/mL

View 49 publications 49 publications

Flow Cytometry (Flow)

1 µg/mL

View 7 publications 7 publications

ELISA (ELISA)

2-10 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

-

View 3 publications 3 publications

Neutralization (Neu)

-

View 1 publication 1 publication

Inhibition Assays (IA)

100 µg/mL

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

-

View 96 publications 96 publications

Product Specifications

Species Reactivity

Guinea pig, Human

Published species

Avian, Bovine, Dog, Fruit fly, Human, Insect, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

HECD-1

Immunogen

Human breast tumor cell line MCF-7.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

2.0 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533003

Product Specific Information

Source: Monoclonal antibody was obtained by fusing the P3-X63-Ag8-U1 mouse myeloma cell line with spleen cells of BALB/c mouse after immunization with human breast tumor cell line MCF-7.

Specificity: This antibody specifically reacts with human E-cadherin. This antibody inhibits E-cadherin dependent cell-cell contact.

Cross reactivity: This antibody reacts with guinea pig E-cadherin. This antibody does not react with bovine, dog, rat or chicken E-cadherin.

Storage: 4°C This product does not contain preservative. The stock solution (2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles. Diluted antibody should not be stored.

Reconstitution: Dissolve the lyophilized antibody in 50 µL of distilled water (final concentration: 2.0 mg/mL). This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 µL or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/mL. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.

Ca2+ should be contained in all the buffers to stabilize cadherin antigen.

Target Information

E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.