Thermo Fisher Scientific MAP2 Monoclonal Antibody (M13)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 7 publications 7 publications

Immunohistochemistry (IHC)

5-10 µg/mL

View 10 publications 10 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:250-1:400

View 35 publications 35 publications

Flow Cytometry (Flow)

-

View 3 publications 3 publications

ELISA (ELISA)

0.1-0.5 µg/mL

View 2 publications 2 publications

Immunoprecipitation (IP)

2-5 µg

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Horse, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

M13

Immunogen

MAPs

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533001

Product Specific Information

This antibody reacts with a 300 kD cytoskeletal protein (MAP2A and MAP2B) primarily expressed in the somatodendritic domain of the neuron but is also found in the glia. Reactivity is not affected by phospohorylation state of MAP2. Abnormal expression of MAP2 may cause cytoskeletal abnormalities and impair signal transduction in dendrites.

This antibody is suitable for immunohistochemical staining of alcohol-fixed paraffin embedded or frozen tissue sections.

Target Information

MAP2 (Microtubule Associated Protein 2) exists in two high molecular weight forms (MAP2a & MAP2b) and a low molecular weight form. The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene. MAP2 is involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dendrites, implicating a role in determining and stabilizing dendritic shape during neuron development. MAP2 is useful in studies of neuron structure in normal and malignant brain tissue as well as in degenerative diseases such as Alzheimer`s Disease (AD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.