Thermo Fisher Scientific MAP2 Monoclonal Antibody (M13)
MAP2 단백질을 인식하는 M13 클론 단일클론 항체로, 신경세포의 수상돌기 영역을 표지하는 연구용 시약. WB, IHC, ICC, ELISA 등 다양한 응용에 적합하며 인간, 마우스, 랫트 반응성. 액상 형태로 -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | 7 |
| Immunohistochemistry (IHC) | 5–10 µg/mL | 10 |
| Immunohistochemistry (Frozen) (IHC (F)) | – | 1 |
| Immunocytochemistry (ICC/IF) | 1:250–1:400 | 35 |
| Flow Cytometry (Flow) | – | 3 |
| ELISA | 0.1–0.5 µg/mL | 2 |
| Immunoprecipitation (IP) | 2–5 µg | 1 |
| Miscellaneous PubMed (Misc) | – | 2 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Horse, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M13 |
| Immunogen | MAPs |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533001 |
Product Specific Information
This antibody reacts with a 300 kD cytoskeletal protein (MAP2A and MAP2B) primarily expressed in the somatodendritic domain of neurons and also found in glia. Reactivity is not affected by the phosphorylation state of MAP2. Abnormal expression of MAP2 may lead to cytoskeletal abnormalities and impaired dendritic signal transduction.
This antibody is suitable for immunohistochemical staining of alcohol-fixed paraffin-embedded or frozen tissue sections.
Target Information
MAP2 (Microtubule Associated Protein 2) exists in two high molecular weight forms (MAP2a & MAP2b) and a low molecular weight form. Expression is developmentally regulated, and multiple forms arise by alternative splicing of a single gene. MAP2 is involved in microtubule assembly, an essential step in neurogenesis. In rat and mouse, MAP2 products are neuron-specific cytoskeletal proteins enriched in dendrites, playing a role in determining and stabilizing dendritic shape during neuron development.
MAP2 is useful in studies of neuron structure in normal and malignant brain tissue as well as in degenerative diseases such as Alzheimer’s Disease (AD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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