Thermo Fisher Scientific Phospho-ERK1/ERK2 (Thr202, Tyr204, Thr185, Tyr187) Recombinant Rabbit Monoclonal Antibody (SC58-01)
Phospho-ERK1/ERK2 (Thr202/Tyr204, Thr185/Tyr187) 인산화 단백질 검출용 항체. Western blot에 최적화된 recombinant rabbit monoclonal 항체로 높은 특이성과 재현성 제공. Human, Mouse, Rat 시료에 반응하며, Protein A 정제 및 1 mg/mL 농도.
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Applications
Western Blot (WB)
- Tested Dilution: 1:5000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SC58-01 |
| Immunogen | Synthetic peptide within Human aa 166–215 (ERK1: UniProt P27361-1, ERK2: UniProt P28482-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% Sodium Azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809480 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned and expressed, and individual clones are screened to select optimal candidates.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to the larger rabbit immune repertoire
Target Information
ERK1 and ERK2 are widely expressed kinases involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. These pathways are activated by various stimuli such as growth factors, cytokines, viral infections, and GPCR ligands. Upon activation, ERKs phosphorylate cytoplasmic targets or translocate to the nucleus to activate transcription factors like c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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