Thermo Fisher Scientific Phospho-Histone H3 (Thr81) Polyclonal Antibody
Histone H3의 Thr81 인산화 형태를 특이적으로 인식하는 폴리클로날 항체로, WB, IHC(P), ICC/IF에 사용 가능. 사람, 생쥐, 랫트 반응성. PBS/glycerol buffer에 보관되며 -20°C에서 안정. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human Histone H3 (Accession P68431), corresponding to amino acid residues around phosphorylated Thr81 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817033 |
Product Specific Information
This antibody detects endogenous levels of Histone H3 only when phosphorylated at Thr81.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, together with four core histone proteins, binds to DNA forming the nucleosome structure. Histones play a central role in transcription regulation, DNA repair, replication, and chromosomal stability. Post-translational modifications of histones, including methylation, acetylation, and phosphorylation, influence chromatin structure and gene expression. The N-terminal tail of histone H3 can undergo multiple modifications that affect cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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