Thermo Fisher Scientific Phospho-Histone H3 (Ser58) Polyclonal Antibody
Histone H3의 Ser58 인산화 형태를 특이적으로 검출하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF에 적합하며, 인산화된 H3 단백질 수준 분석에 유용. 고순도 정제 및 안정적인 PBS/glycerol buffer로 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human Histone H3 (Accession P68431), corresponding to amino acid residues around phosphorylated Ser58 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817031 |
Product Specific Information
Antibody detects endogenous levels of Histone H3 only when phosphorylated at Ser58.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translationally, histones are modified in various ways to either directly change chromatin structure or allow binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the nucleosome core and undergoes modifications such as methylation, acetylation, and phosphorylation, influencing key cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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