Thermo Fisher Scientific Phospho-Ephrin B1 (Tyr313, Tyr317) Polyclonal Antibody
EFNB1의 Tyr313/317 인산화 형태를 특이적으로 검출하는 Rabbit Polyclonal 항체. Western blot 및 IHC(P)에서 사용 가능. 인간, 마우스, 랫트 반응성. 고순도 액상 형태로 -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human EFNB1 (Accession P98172), corresponding to amino acid residues around phosphorylated Tyr313 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817036 |
Product Specific Information
Antibody detects endogenous levels of EFNB1 only when phosphorylated at Tyr313/317.
Target Information
EFNB1 is a cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases crucial for migration, repulsion, and adhesion during neuronal, vascular, and epithelial development. It binds Eph receptors on adjacent cells, leading to bidirectional signaling—forward signaling via the receptor and reverse signaling via the ephrin ligand. EFNB1 interacts with EPHA4, EPHA3, and EPHB4, and together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells and may influence axon orientation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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