Thermo Fisher Scientific Phospho-Histone H3 (Thr12) Polyclonal Antibody
Rabbit polyclonal antibody specific for phosphorylated Histone H3 at Thr12. Validated for WB, IHC(P), and ICC/IF. Detects endogenous levels only when phosphorylated. Suitable for chromatin and transcription regulation studies. For research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human Histone H3 (Accession P68431), corresponding to amino acid residues around phosphorylated Thr12 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817032 |
Product Specific Information
Antibody detects endogenous levels of Histone H3 only when phosphorylated at Thr12.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. Together with four core histone proteins, it binds to DNA forming the nucleosome structure. Histones play a central role in transcription regulation, DNA repair, replication, and chromosomal stability. Post-translational modifications of histones, including methylation, acetylation, and phosphorylation, influence chromatin structure and cellular processes. The N-terminal tail of histone H3 can undergo these modifications, affecting transcription factor binding and chromatin dynamics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일: PA5-105604_Histone_H3_P68431-1_Rabbit.svg, PA5-105604_Histone_H3_Q71DI3-1_Rabbit.svg 등)
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