Thermo Fisher Scientific 14-3-3 zeta Recombinant Rabbit Monoclonal Antibody (1H9L19)
Western blot 및 면역세포화학(ICC/IF)에 적합한 14-3-3 zeta 단백질 인식용 재조합 토끼 단일클론 항체. 높은 특이성과 재현성, 로트 간 일관성 제공. 인간, 마우스, 랫드 등 다양한 종에 반응. 단백질 A로 정제된 액상 형태로 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Cynomolgus monkey, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 1H9L19 |
| Immunogen | Peptides corresponding to human 14-3-3 delta/zeta (aa 68–86, 131–149) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2663061 |
Product Specific Information
This antibody is predicted to react with Pig, Rat, and Horse.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin–free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
The 14-3-3 family comprises at least seven highly conserved isoforms of homo- and heterodimeric proteins expressed in all eukaryotic cells. They function as key regulators of signal transduction events mediated through binding to serine-phosphorylated proteins.
By interacting with Cdc25C, 14-3-3 regulates entry into the cell cycle, and through interaction with Bad, prevents apoptosis. Other known binding partners include protein kinase C family members, Cbl, IRS-1, polyoma middle-T antigen, nitrate reductase, S-raf, and IGF-1 receptor.
Detection of 14-3-3 proteins in cerebrospinal fluid has been useful in the differential diagnosis of Creutzfeldt-Jakob disease and other prion diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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