Thermo Fisher Scientific IGFBP-1 Polyclonal Antibody, Biotin, PeproTech
Biotin으로 표지된 Rabbit Polyclonal Anti-Human IGFBP-1 항체로, Western blot 및 ELISA에 적합합니다. 고순도 재조합 단백질로 면역하여 제작되었으며, 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific IGFBP-1 Polyclonal Antibody, Biotin (PeproTech)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human IGF-BP1 |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | No preservative |
| Storage conditions | -20°C |
| Shipping conditions | Ambient |
Product Specific Information
AA Sequence of recombinant protein:
MAPWQCAPCS AEKLALCPPV SASCSEVTRS AGCGCCPMCA LPLGAACGVA TARCARGLSC RALPGEQQPL HALTRGQGAC VQESDASAPH AAEAGSPESP ESTEITEEEL LDNFHLMAPS EEDHSILWDA ISTYDGSKAL HVTNIKKWKE PCRIELYRVV ESLAKAQETS GEEISKFYLP NCNKNGFYHS RQCETSMDGE AGLCWCVYPW NGKRIPGSPE IRGDPNCQIY FNVQN
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IGF-BP1. Anti-Human IGF-BP1-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect hIGF-BP1 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IGF-BP1 (500-P228) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hIGF-BP1.
Western Blot:
To detect hIGF-BP1 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant hIGF-BP1 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Package Information:
500-P228BT-1MG will be provided as 2 × 500 µg.
Target Information
This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명: 500-P228BT-500UG_IGFBP-1_P08833-1_Rabbit.svg, 500-P228BT-500UG_IGFBP-1_P08833-1_Rabbit_PDP.jpeg)
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