Thermo Fisher Scientific IGFBP-1 Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific IGFBP-1 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MAPWQCAPCS AEKLALCPPV SASCSEVTRS AGCGCCPMCA LPLGAACGVA TARCARGLSC RALPGEQQPL HALTRGQGAC VQESDASAPH AAEAGSPESP ESTEITEEEL LDNFHLMAPS EEDHSILWDA ISTYDGSKAL HVTNIKKWKE PCRIELYRVV ESLAKAQETS GEEISKFYLP NCNKNGFYHS RQCETSMDGE AGLCWCVYPW NGKRIPGSPE IRGDPNCQIY FNVQN

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IGF-BP1. Anti-Human IGF-BP1-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hIGF-BP1 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IGF-BP1 (500-P228) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hIGF-BP1.

Western Blot:
To detect hIGF-BP1 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hIGF-BP1 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P228BT-1MG will be provided as 2 × 500 µg.

Target Information

This gene belongs to the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with IGFBP and thyroglobulin type-I domains. The protein binds both IGF-I and IGF-II and circulates in plasma, prolonging IGF half-life and modulating receptor interactions.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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