Thermo Fisher Scientific Glypican 3 Monoclonal Antibody (9C2)
Glypican 3 단백질 검출용 Mouse monoclonal antibody (Clone 9C2). Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 응용에 사용 가능. Human 및 Mouse 시료 반응. Protein G 정제, 1 mg/mL 농도, PBS buffer에 sodium azide 포함.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (IHC) | – | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 | View 1 publication |
| Flow Cytometry (Flow) | 1:200–1:400 | – |
| ELISA | 1:10,000 | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9C2 |
| Immunogen | Purified recombinant fragment of human GPC3 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4 °C short term; for long-term storage, store at −20 °C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538554 |
Product Specific Information
MA5-17083 targets GPC3 in indirect ELISA, FACS, ICC, IHC, IF, and WB applications, showing reactivity with Human and Mouse samples.
The immunogen is a purified recombinant fragment of human GPC3 expressed in E. coli.
MA5-17083 detects GPC3, which has a predicted molecular weight of approximately 65.5 kDa.
Target Information
GPC3 is a cell surface proteoglycan bearing heparan sulfate. It may be involved in suppression or modulation of growth in mesodermal tissues and organs, and may modulate IGF2 interactions with its receptor. Members of the glypican-related proteoglycan family are anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol (GPI) linkage, playing roles in cell division, growth regulation, and tumor predisposition.
Deletion mutations in GPC3 cause Simpson-Golabi-Behmel syndrome (SGBS), characterized by pre- and postnatal overgrowth with visceral and skeletal anomalies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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