Thermo Fisher Scientific Calnexin Polyclonal Antibody
Calnexin 단백질을 검출하기 위한 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, 다양한 종에 반응하며 WB, IHC, ICC/IF, Flow, IP 등 여러 응용에 사용 가능. 고순도 Protein A 및 항원 친화 크로마토그래피로 정제된 액상 시약.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | Assay-Dependent |
| Immunoprecipitation (IP) | Assay-Dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Avian, Bovine, Dog, Chicken, Chimpanzee, Fruit fly, Guinea pig, Hamster, Human, Mouse, Sheep, Pig, Rabbit, Rat, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Dog Calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735568 |
Product Specific Information
A 1:2,000 dilution of PA5-77839 was sufficient for detection of Calnexin in 10 µg of HeLa cell lysate by ECL immunoblot analysis. Detects the C-terminal domain of Calnexin (~90 kDa). Weak detection observed in Chicken, Drosophila, and Xenopus tissues.
Target Information
Calnexin (also known as IP90, p88, p90) is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER). It functions as a molecular chaperone, aiding in the proper folding and assembly of polypeptides. Calnexin associates with MHC class I heavy chains, partial complexes of T cell receptors, and B cell membrane immunoglobulins, but not with completed receptor complexes. It retains incompletely folded proteins in the ER. Unlike soluble ER resident proteins that contain the KDEL sequence, calnexin lacks this sequence but has positively charged cytosolic residues ensuring ER retention. The protein includes a 461-amino-acid luminal domain, a 22-amino-acid transmembrane segment, and an 89-amino-acid cytoplasmic tail.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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