Thermo Fisher Scientific IL-15 Polyclonal Antibody, PeproTech

Applications and Tested Dilutions

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MNWIDVRYDL EKIESLIQSI HIDTTLYTDS DFHPSCKVTA MNCFLLELQV ILHEYSNMTL NETVRNVLYL ANSTLSSNKN VAESGCKECE ELEEKTFTEF LQSFIRIVQM FINTS.

Preparation:
Produced from sera of rabbits immunized with highly pure recombinant murine IL-15. Anti-murine IL-15-specific antibody was purified by affinity chromatography employing an immobilized murine IL-15 matrix.

Sandwich ELISA:
To detect murine IL-15 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen-affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IL-15 (500-P173Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of recombinant murine IL-15.

Western Blot:
To detect murine IL-15 by Western blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for recombinant murine IL-15 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Target Information

IL-15 (Interleukin 15) is a cytokine that regulates T and natural killer (NK) cell activation and proliferation. IL-15 and IL-2 share many biological activities as both bind common hematopoietin receptor subunits, potentially competing for the same receptor and negatively regulating each other's activity. The number of CD8+ memory cells is controlled by a balance between IL-15 and IL-2.

IL-15 induces activation of JAK kinases and phosphorylation of transcription activators STAT3, STAT5, and STAT6. In mouse models, IL-15 may increase expression of apoptosis inhibitor Bcl-xL, possibly through STAT6 activation, preventing apoptosis. IL-15 plays a key role in the growth and differentiation of T and B lymphocytes, NK cells, macrophages, and monocytes, as well as activation of intracellular signaling molecules.

It is essential for immune responses, allograft rejection, and autoimmune disease pathogenesis. IL-15 is a pro-inflammatory cytokine implicated in disorders such as rheumatoid arthritis, psoriasis, and pulmonary inflammation. Neutralization of IL-15 shows potential therapeutic benefits in models of psoriasis and diabetes.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.