Thermo Fisher Scientific IL-15 Polyclonal Antibody, Biotin, PeproTech
Biotin으로 결합된 Rabbit Polyclonal Anti-Murine IL-15 항체로, Western blot, ELISA, Flow cytometry 등에 사용 가능. IL-15 관련 면역 반응 연구에 적합하며, 높은 특이성과 민감도를 제공. 항원 친화 크로마토그래피로 정제된 연구용 시약.
- 카탈로그번호
- 500-P173BT-xxxx (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-15 Polyclonal Antibody, Biotin, PeproTech
Thermo Fisher Scientific IL-15 Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | – |
| Flow Cytometry (Flow) | – | 6 publications |
| ELISA | 0.25–1.0 µg/mL | 1 publication |
| In vitro Assay (IV) | – | 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Murine IL-15 |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929633 |
Product Specific Information
AA Sequence of recombinant protein:
MNWIDVRYDL EKIESLIQSI HIDTTLYTDS DFHPSCKVTA MNCFLLELQV ILHEYSNMTL NETVRNVLYL ANSTLSSNKN VAESGCKECE ELEEKTFTEF LQSFIRIVQM FINTS
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-15. Anti-Murine IL-15-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect mIL-15 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IL-15 (500-P173) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant mIL-15.
Western Blot:
To detect mIL-15 by Western Blot, use at a concentration of 0.1–0.2 µg/mL. With compatible secondary reagents, the detection limit for Recombinant mIL-15 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Packaging:
500-P173BT-1MG is provided as 2 × 500 µg.
Target Information
IL-15 (Interleukin 15) is a cytokine that regulates T and natural killer cell activation and proliferation. IL-15 and IL-2 share common hematopoietin receptor subunits and may compete for the same receptor, thus negatively regulating each other’s activity.
IL-15 induces activation of JAK kinases and phosphorylation of STAT3, STAT5, and STAT6, affecting apoptosis inhibition through Bcl-xL expression.
It plays a crucial role in the growth and differentiation of T and B lymphocytes, NK cells, macrophages, and monocytes, and is involved in immune responses, allograft rejection, and autoimmune disease pathogenesis.
IL-15 is also implicated in inflammatory disorders such as rheumatoid arthritis, psoriasis, and pulmonary inflammation. Neutralization of IL-15 has shown beneficial effects in models of psoriasis and diabetes.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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