Thermo Fisher Scientific EMAP II Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

• AA Sequence of recombinant protein: discontinued
• Preparation: Produced from sera of goats immunized with highly pure Recombinant Human EMAP-II. Anti-Human EMAP-II-specific antibody was purified by affinity chromatography and then biotinylated.
• Sandwich ELISA: To detect hEMAP-II by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human EMAP-II (500-P172G) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hEMAP-II.
• Western Blot: To detect hEMAP-II by Western Blot analysis, this antibody can be used at 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hEMAP-II is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
• Package: 500-P172GBT-1MG is provided as 2 × 500 µg.

Target Information

The protein encoded by this gene is a cytokine specifically induced by apoptosis and is involved in the control of angiogenesis, inflammation, and wound healing. Its release renders tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit associated with the multi-tRNA synthetase complex and modulates aminoacylation activity of tRNA synthetase in normal cells. It also stimulates inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found, and a pseudogene has been identified on chromosome 20.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.