Norgen Saliva DNA Isolation Kit

Norgen Saliva DNA Isolation Kit

SKU: RU45400
Rapid purification of high-quality DNA from preserved and fresh saliva samples.

---

Features and Benefits

• Fast and easy processing
• Isolates high-quality genomic DNA from fresh or preserved saliva samples
• Available in multiple formats to suit different throughput needs
• Compatible with preserved saliva samples collected using Norgen’s Saliva DNA Collection and Preservation Devices (Cat. RU49000)

Saliva DNA purified using this kit is suitable for downstream applications such as PCR, Southern blotting, sequencing, and microarray analysis.

---

Available Formats

Format	Catalog No.	Description
Spin Column	RU45400	Simple spin column method for genomic DNA isolation
High Throughput	RU35200	96-well format for rapid, high-yield DNA isolation
Magnetic Bead System	RU55400, RU62900	Magnetic bead-based DNA isolation, compatible with automated systems (IsoPure™, KingFisher, Hamilton Star/Vantage, Tecan)

---

Background

Saliva is a non-invasive alternative to blood collection for genomic DNA extraction. DNA from buccal epithelial cells and leukocytes in saliva can be used for disease diagnosis, monitoring, and infection detection.
Norgen’s Saliva DNA Isolation Kit enables fast and reliable DNA isolation from both preserved and fresh saliva samples.

---

Supporting Data

Figure 1. High Quality and Yield of DNA from Saliva Samples

Total DNA was isolated from 250 µL of eight fresh saliva samples using Norgen’s kit.
For evaluation, 10 µL of each 100 µL elution was run on a 1.2% agarose gel.
High yield and quality were observed in all lanes.
Lane M: Norgen’s FastRunner 1 kb DNA Ladder.

Gel Lane Labels

Lane	Label
1	M
2	1
3	2
4	3
5	4
6	5
7	6
8	7
9	8
10	M

Figure 2. Real-time PCR Consistency from Saliva Samples

DNA was isolated from 250 µL of fresh saliva (10 samples from one donor).
5 µL of each elution was used in real-time PCR for GAPDH amplification.
Consistent Ct values confirmed reproducibility and high DNA quality.

Amplification Curve Data

• Graph Title: Amplification
• X-axis: Cycles (0–40)
• Y-axis: RFU (0–1600)

Figure 3. Illumina MiSeq 16s rRNA Metagenomics Data

DNA isolated from saliva preserved for up to 6 years at room temperature.

Section	Title	Description
A	Principal Coordinate Analysis (PCoA)	PCoA plot showing sample distribution (coordinates 1–14)
B	Hierarchical Clustering Dendrogram	Cluster analysis with genus-level abundance visualization

Figure 4. Consistent DNA Isolation

Samples from 10 donors were pooled and processed using column and 96-well formats.
Consistent DNA isolation observed across all lanes.

Gel Lane Labels

Lane	Label
1	M
2	S
3	S
4–23	1–20
24	M

Figure 5. Real-time PCR Consistency (RU35200)

PCR amplification curves comparing 96-well and column formats.
GAPDH gene successfully amplified with no inhibition.

Graph Data

• Y-axis: PCR Base Line Subtracted CF RFU
• X-axis: Cycle
• Title: PCR Amplification vs Cycle (23-Sep-11 1114.opd)

---

Kit Specifications

Specification	Description
Maximum Saliva Input	0.5 mL preserved saliva / 0.25 mL fresh saliva
Average Yield (0.25 mL saliva)*	3–7 µg
Average Purity (OD260/280)	1.7–2.1
Time for 10 Purifications	30 minutes

* Yield may vary depending on donor.

---

Storage and Stability

All solutions should be tightly sealed and stored at room temperature.
Stable for 1 year post-shipment.
Proteinase K in storage buffer remains stable for 1 year at room temperature.

---

Kit Components

Component	RU45400 (50 preps)	RU35200 (192 preps)	RU55400 (50 preps)	RU62900 (192 preps)
Lysis Buffer F	30 mL	100 mL	30 mL	60 mL
Proteinase K	1.2 mL	4 mL	1.2 mL	4 mL
Magnetic Beads A	–	–	2.2 mL	8.5 mL
Solution WN	–	–	18 mL	55 mL
Binding Buffer B	12 mL	40 mL	–	–
Wash Solution A	18 mL	2×38 mL	–	–
Elution Buffer B	15 mL	30 mL	8 mL	30 mL
Spin Columns	50	–	–	–
96-Well Plate	–	2	–	2
Collection Tubes	50	–	–	–
Adhesive Tape	–	4	–	2
Product Insert	1	1	1	1

---

Automation

Automation scripts available for integration with robotic systems.
Contact Norgen for protocol access.

---

Documentation

• RU45400 Protocol (50 prep)
• RU35200 Protocol (96-well)
• RU55400 Protocol (Magnetic Bead 50 prep)
• RU62900 Protocol (Magnetic Bead 96-well)

---

FAQs (Selected)

Q. What to do if column gets clogged?
Ensure proper centrifugation speed/time, apply ≤0.5 mL saliva, and vortex lysate before loading.

Q. Why low DNA yield?
May result from incomplete lysis or low DNA concentration in sample; extend Proteinase K incubation.

Q. Poor downstream performance?
Ensure thorough washing and removal of ethanol before elution.

Q. RNA contamination?
Perform RNase A digestion if RNA interferes with downstream use.

Q. Can process swab or non-human saliva?
Yes, with supplementary protocol; validated on canine samples.

---

제품 이미지