Thermo Fisher Scientific Phospho-ZAP70/Syk (Tyr319, Tyr352) Recombinant Rabbit Monoclonal Antibody (Zap70Y319-A11)
ZAP-70/Syk 단백질의 Tyr319 및 Tyr352 인산화 부위를 특이적으로 인식하는 재조합 토끼 단클론 항체입니다. Western blot에 적합하며, 높은 특이성과 재현성을 제공합니다. 동물 유래 성분이 없고, 로트 간 일관성이 우수합니다.
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Applications
- Western Blot (WB): 1–1,000 ng/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | Zap70Y319-A11 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Tyr319/Tyr352 of human phospho Zap70/Syk |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2745061 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Specific antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates for production.
These antibodies provide:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase belonging to the Syk family, expressed in T-cells and natural killer cells. It is essential for T-cell receptor activation.
Upon TCR engagement, ZAP70 is phosphorylated at tyrosines 292, 315, and 319.
- Tyr315 (Vav binding site) and Tyr319 (Lck binding site) enhance signaling
- Tyr292 phosphorylation attenuates activation
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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