Thermo Fisher Scientific Phospho-ZAP70/Syk (Tyr493, Tyr526) Recombinant Rabbit Monoclonal Antibody (Zap70Y493-H11), PE
ZAP70/Syk 인산화(Tyr493, Tyr526) 검출용 PE-conjugated recombinant rabbit monoclonal antibody. 높은 특이성과 재현성을 제공하며, Flow Cytometry에 최적화되어 있습니다. 연구용으로만 사용 가능합니다.
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Applications
Flow Cytometry (Flow)
- Tested Dilution: 5 µL / 1×10⁶ cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG, kappa |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | Zap70Y493-H11 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Tyr493 of human phospho Zap70 |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Storage Conditions | 2–8°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2745063 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase belonging to the Syk family. It is expressed exclusively in T-cells and natural killer cells and is essential for T-cell receptor activation.
Upon T-cell receptor engagement, ZAP70 is phosphorylated on tyrosines 292, 315, and 319.
- Tyr315 (Vav binding site) and Tyr319 (Lck binding site) phosphorylation enhances ZAP70 function in lymphocyte signaling.
- Tyr292 phosphorylation terminates transient activation and attenuates signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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