Thermo Fisher Scientific Phospho-ZAP70/Syk (Tyr319, Tyr352) Recombinant Rabbit Monoclonal Antibody (Zap70Y319-A3)
Human 및 Mouse 시료에서 ZAP-70/Syk 인산화 형태(Tyr319, Tyr352)를 검출하는 재조합 Rabbit Monoclonal 항체. 높은 특이성과 일관된 로트 간 재현성을 제공하며, Flow Cytometry에 적합. PBS buffer에 50% glycerol 포함, -20°C 보관.
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Thermo Fisher Scientific Phospho-ZAP70/Syk (Tyr319, Tyr352) Recombinant Rabbit Monoclonal Antibody (Zap70Y319-A3)
Applications
- Flow Cytometry (Flow)
Tested Dilution
- 1–1,000 ng/mL
Species Reactivity
- Human
- Mouse
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | Zap70Y319-A3 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Tyr319/Tyr352 of human phospho Zap70/Syk |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2745060 |
Additional Formats
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened for optimal production.
Advantages:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to large rabbit immune repertoire
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase in the Syk family, expressed in T-cells and natural killer cells. It is essential for T-cell receptor activation. Upon TCR engagement, ZAP70 is phosphorylated at tyrosines 292, 315, and 319.
Phosphorylation at Tyr315 (Vav binding site) and Tyr319 (Lck binding site) enhances ZAP70-mediated lymphocyte signaling, while Tyr292 phosphorylation attenuates activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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