Thermo Fisher Scientific MDM2 Polyclonal Antibody
MDM2 단백질을 표적하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체로, WB, IHC, IP, ChIP 등 다양한 응용에 적합합니다. 인간 및 생쥐 반응성, 고순도 항원 친화 크로마토그래피 정제, 장기 보관 시 -20°C에서 안정적 유지.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific MDM2 Polyclonal Antibody
Thermo Fisher Scientific MDM2 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:500 |
| ChIP assay (ChIP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MDM2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.68 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792242 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Rat (81%), Dog (91%), Cat (95%), Pig (93%), Rhesus Monkey (98%), Bovine (95%).
- Positive Control: Jurkat, Raji, NCI-H929, Neuro2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, HepG2, HepG2 (starvation and 1 µM Staurosporine treatment for 24 hr), MDM2-transfected 293T.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
MDM2 is a ubiquitin ligase for p53 and plays a central role in regulation of p53 stability. Located on chromosome 12q, MDM2 is phosphorylated by Akt at Ser166 and Ser186, enhancing its interaction with p300 and promoting p53 ubiquitination and degradation. Phosphorylation also inhibits MDM2 binding to p19ARF, further increasing p53 degradation. MDM2 negatively regulates p53 by inhibiting its transactivation and promoting its degradation via E3 ubiquitin ligase activity. Overexpression of MDM2 leads to p53 inactivation and reduced tumor suppressor function. MDM2 is implicated in apoptosis, cell cycle regulation, and is overexpressed in various malignancies including soft tissue carcinomas and breast cancer. It interacts with proteins such as retinoblastoma 1 and ribosomal protein L5, and more than 40 alternatively spliced variants have been identified in both tumor and normal tissues.
Additional Information
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.