Thermo Fisher Scientific CD69 Monoclonal Antibody (H1.2F3), Functional Grade, eBioscience
마우스 CD69 항원 인식용 아르메니아 햄스터 유래 단클론 항체로, 림프구 활성화 연구에 적합합니다. Flow Cytometry 및 세포 활성화 실험에 사용 가능하며, 1 mg/mL 농도의 액상 형태로 제공됩니다. 4°C에서 보관하며, 높은 순도와 낮은 엔도톡신 수준을 보장합니다.
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Thermo Fisher Scientific CD69 Monoclonal Antibody (H1.2F3), Functional Grade, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 0.5 µg/test | View 57 publications |
| ELISA | - | View 1 publication |
| Functional Assay | Assay-Dependent | View publications |
Product Specifications
| Specification | Description |
|---|---|
| Published Species | Not Applicable |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | H1.2F3 |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2865783 |
Available Conjugate Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 496
- Brilliant UV 661
- Brilliant UV 737
- Brilliant Violet 421
- Brilliant Violet 786
- eFluor 450
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 (~35 kDa) is expressed on the surface as a disulfide-linked dimer. Activation of T and B cells rapidly upregulates CD69 expression, suggesting a role in lymphocyte development and activation.
Applications Reported:
- Flow cytometric analysis
- In vitro cell activation assays
Applications Tested:
Tested by flow cytometric analysis of resting and activated mouse splenocytes. Recommended usage ≤ 0.5 µg/test in 100 µL final volume. Optimal titration should be performed for specific assays.
Storage and Handling:
Use in a sterile environment.
Filtration:
0.2 µm post-manufacturing filtered.
Purity:
90%, determined by SDS-PAGE.
Endotoxin Level:
<0.001 ng/µg antibody (LAL assay).
Aggregation:
<10%, determined by HPLC.
Target Information
CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide-bonded homodimer (~60 kDa under non-reducing conditions). It contains N-linked oligosaccharides and is present on activated platelets. CD69 is involved in lymphocyte signal transduction and proliferation, and may transmit signals in natural killer cells and platelets. Alternative splicing produces multiple transcript variants.
Diseases associated with CD69 dysfunction include coccidioidomycosis and asthma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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