Thermo Fisher Scientific CD64 Monoclonal Antibody (10.1), Functional Grade, eBioscience

Thermo Fisher Scientific CD64 Monoclonal Antibody (10.1), Functional Grade, eBioscience™

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• eFluor 450
• FITC
• NovaFluor Blue 660-120S
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

Description:
The 10.1 monoclonal antibody reacts with human CD64 (FcγRI), a 75 kDa type I transmembrane protein. CD64 is the high-affinity receptor for IgG and is involved in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, and cytokine production regulation. Monocytes and macrophages express CD64, while mature granulocytes and lymphocytes are negative.

Applications Reported:

• Flow cytometric analysis
• Immunohistology staining of frozen tissue sections

Applications Tested:
Tested by flow cytometric analysis of normal human peripheral blood cells. Recommended use ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Antibody titration is advised for optimal performance.

Storage and Handling:
Use in a sterile environment.

Filtration:
0.2 µm post-manufacturing filtered.

Purity:

90% by SDS-PAGE.

Endotoxin Level:
<0.001 ng/µg antibody (LAL assay).

Aggregation:
<10% by HPLC.

Target Information

CD64 (FCGR1A) encodes a high-affinity Fc-gamma receptor protein, one of three related gene family members located on chromosome 1. It plays a critical role in immune response, with dysfunctions studied in cervical adenitis and tetrasomy 21.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.