Thermo Fisher Scientific CD70 Monoclonal Antibody (FR70), Functional Grade, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 4 publications 4 publications

Neutralization (Neu)

Assay-Dependent

-

Functional Assay (Functional)

Assay-Dependent

View 2 publications 2 publications

Product Specifications

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

FR70

Conjugate

Functional Grade Functional Grade Functional Grade

View additional formats

• Unconjugated
• APC
• PE
• PerCP-eFluor 710
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2865786

Product Specific Information

Description: The FR70 monoclonal antibody reacts with mouse CD70, a 30-33 kDa type II transmembrane glycoprotein. CD70 is a member of the TNF family and is expressed by activated mouse T and B lymphocytes. Interaction of CD70 with CD27, its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.

Applications Reported: The FR70 antibody has been reported for use in flow cytometric analysis. FR70 has also been reported in blocking of CD70 in vitro .

Applications Tested: The FR70 antibody has been tested by flow cytometric analysis of LPS-stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.