Thermo Fisher Scientific CD73 (Immuno-Oncology Target) Monoclonal Antibody (NT5E, 2545)
인간 CD73(NT5E)에 특이적인 마우스 단클론 항체로, 면역온콜로지 연구에 적합. WB, IHC, ICC/IF, Flow Cytometry 등 다양한 응용 가능. Recombinant full-length NT5E를 면역원으로 사용. 단백질 A/G로 정제된 액상 형태, 4°C 보관.
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Thermo Fisher Scientific CD73 (Immuno-Oncology Target) Monoclonal Antibody (NT5E, 2545)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | NT5E, 2545 |
| Immunogen | Recombinant full-length human NT5E protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Formalin-fixed tissues require heating in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
CD73 (Ecto-5'-nucleotidase, 5'-ribonucleotide phosphohydrolase) catalyzes the conversion of purine 5'-mononucleotides to nucleosides at neutral pH, with AMP as the preferred substrate.
It is a dimer of two identical 70 kDa subunits bound externally to the plasma membrane via a glycosyl phosphatidyl inositol linkage.
CD73 serves as a marker of lymphocyte differentiation, and its deficiency is linked to immunodeficiency diseases.
Other 5'-nucleotidase forms exist in cytoplasm and lysosomes, distinguishable by substrate affinity and cofactor requirements.
The CD73 gene is located on chromosome 6q14–q21. Mutations can lead to joint and arterial calcification and intestinal tuberculosis.
Two transcript variants encoding distinct isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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