Thermo Fisher Scientific CD73 (Immuno-Oncology Target) Monoclonal Antibody (NT5E, 2505)
인간 CD73 단백질을 인식하는 Mouse IgG1 단클론 항체로, 면역항암 연구용으로 적합. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용 가능. 단백질 A/G로 정제된 액상 형태이며 보존제 무첨가. -20°C 또는 -80°C 보관 권장.
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- 4907-MSM3-P1ABX
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Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | NT5E, 2505 |
| Immunogen | Recombinant full-length human NT5E protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage conditions | -20°C or -80°C if preferred |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
CD73 (Ecto-5-prime-nucleotidase, 5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion of purine 5-prime mononucleotides to nucleosides at neutral pH, with AMP as the preferred substrate.
CD73 is a dimer of two identical 70 kDa subunits anchored to the plasma membrane via a glycosyl phosphatidyl inositol linkage. It serves as a marker of lymphocyte differentiation, and its deficiency is associated with various immunodeficiency diseases.
Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and differ from CD73 by substrate affinities, magnesium ion requirements, ATP activation, and phosphate inhibition.
The CD73 gene is localized to chromosome 6q14–q21. Defects in this gene can lead to joint and arterial calcification, as well as intestinal tuberculosis. Two transcript variants encoding different isoforms of CD73 have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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