Thermo Fisher Scientific CD73 (Immuno-Oncology Target) Monoclonal Antibody (NT5E, 2545)
CD73 단백질을 표적으로 하는 인간 반응성 마우스 단클론 항체. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 사용 가능. Protein A/G 정제, 4°C 보관. 면역항암 연구 및 면역세포 표지에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | NT5E, 2545 |
| Immunogen | Recombinant full length human NT5E protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For IHC (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- For formalin-fixed tissues: Heat tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, then cool at room temperature for 20 minutes.
Target Information
CD73 (Ecto-5'-nucleotidase, 5'-ribonucleotide phosphohydrolase) catalyzes the conversion of purine 5'-mononucleotides to nucleosides at neutral pH, with AMP as the preferred substrate.
It forms a dimer of two identical 70 kDa subunits attached to the plasma membrane via a glycosyl phosphatidylinositol linkage.
CD73 serves as a marker of lymphocyte differentiation, and its deficiency is associated with various immunodeficiency diseases.
Defects in the CD73 gene can cause calcification of joints and arteries and intestinal tuberculosis.
The gene is localized to chromosome 6q14–q21, and two transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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