Thermo Fisher Scientific Phospho-Syk (Tyr348) Polyclonal Antibody
Phospho-Syk (Tyr348) 폴리클로날 항체로, 인간·마우스·원숭이·랫트 반응성. Western blot, IHC, ELISA에 사용 가능. 항원 친화 크로마토그래피로 정제된 고순도 항체로, PBS/glycerol 버퍼에 보관. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | 1:100–1:300 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthesized peptide derived from human Syk around the phosphorylation site of Y348 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2608717 |
Product Specific Information
This antibody is predicted to react with human, rat, and monkey based on sequence homology.
Target Information
Spleen tyrosine kinase (SYK), along with ZAP70, are cytoplasmic tyrosine kinases that are viewed as potential drug targets for allergic disorders and autoimmune diseases. SYK may also play a role in cancer.
Syk translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes.
Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motifs of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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