Thermo Fisher Scientific TNF alpha Polyclonal Antibody, eBioscience

Applications

Tested Dilution

Publications

ELISA (ELISA)

-

View 5 publications 5 publications

Functional Assay (Functional)

Assay-Dependent

-

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_493955

Product Specific Information

Description: The affinity purified polyclonal rabbit antibody reacts with mouse and rat tumor necrosis factor-alpha. Mouse TNF alpha is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4 T cells, and NK cells. A 26 kDa form is expressed as a membrane-bound molecule. TNF alpha is cytolytic and plays an important role in immune regulation. Bioactive TNF alpha exists as a homotrimer.

Applications Reported: This affinity purified anti-mouse/rat TNF alpha antibody has been reported for use as the plate-coating (ELISA Capture) antibody for the In Vivo Cytokine Capture Assay (IVCCA), in conjunction with the Functional Grade Biotinylated TN3.19 antibody for In Vivo Capture (ELISA Detection). The IVCCA facilitates measurement of cytokines in serum by increasing their in vivo half lives dramatically. This increases the sensitivity of measurement of in vivo cytokine production 30- to 1000- fold. For detailed methodology in this application, please refer to this reference: Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New York..

Applications Tested: The Affinity Purified anti-mouse/rat TNF alpha antibody has been tested as the plate coating ELISA capture antibody in a sandwich ELISA for analysis of mouse TNF-a, in combination with the Functional Grade Biotinylated anti-mouse TNF alpha antibody (14-8321) for ELISA detection and recombinant mouse TNF-a (14-8321) as the standard. A suitable range of concentrations of the Affinity Purified anti-mouse/rat TNF alpha antibody for ELISA capture is 1.0 - 4.0 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15.0 pg/mL should be included in each ELISA plate.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.