Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MAb11), eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Flow Cytometry (Flow)

Assay-Dependent

View 55 publications 55 publications

ELISA (ELISA)

-

View 3 publications 3 publications

Neutralization (Neu)

-

View 1 publication 1 publication

Functional Assay (Functional)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Cynomolgus monkey, Human, Mouse, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

MAb11

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 488
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 650
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468489

Product Specific Information

Description: The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TN alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.

Applications Reported: MAb11 has been reported for use in detection of human TNF alpha by ELISA. It can also be used for intracellular flow cytometric analysis.

Applications Tested: The biotinylated format for this lot of MAb11 has been tested by sandwich ELISA for the detection of human TNF alpha in combination with MAb1 and can be used at 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.