Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MAb1), eBioscience
인간 TNF-alpha를 인식하는 단클론 항체로, ELISA, 중화, 기능 분석 등에 사용. 0.5 mg/mL 액상 형태로 제공되며, PBS(pH 7.2)에 0.09% sodium azide 포함. 4°C 보관, 인간 및 비인간 영장류 반응성. 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | - | 3 |
| ELISA | 1–4 µg/mL | 6 |
| Neutralization | - | 2 |
| Functional Assay | - | 1 |
| Miscellaneous (PubMed) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Cynomolgus monkey, Human, Non-human primate |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MAb1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_468487 |
Additional Formats
Product Specific Information
Description:
The MAb1 antibody reacts with human tumor necrosis factor-alpha (TNF-alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells, and CD4⁺ T cells. TNF-alpha has cytolytic activity against various tumor cells and plays a crucial role in immune regulation. It forms dimers and trimers and also exists as a 26 kDa membrane-bound form.
Applications Reported:
MAb1 has been reported for use in ELISA capture of human TNF-alpha, immunoblotting (WB), and neutralization of TNF-alpha activity. For functional assays, use the Functional Grade purified MAb1 (Product #16-7348).
Applications Tested:
MAb1 was tested as the capture antibody in a sandwich ELISA with biotin MAb11 (13-7349) as detection and recombinant human TNF-alpha (14-8329) as the standard. Recommended ELISA capture range: 1–4 µg/mL.
A standard curve using recombinant TNF-alpha (1000 pg/mL–8 pg/mL) should be included in each ELISA plate.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
TNF-alpha is a multifunctional proinflammatory cytokine belonging to the TNF superfamily. It is mainly secreted by macrophages and binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR.
It regulates immune response, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF-alpha exists as multimers (dimers, trimers, or pentamers) and appears as a single 17 kDa band under non-reducing SDS-PAGE conditions.
It plays roles in tumor necrosis, immune defense against pathogens, and has implications in autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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