Thermo Fisher Scientific IL-38 Monoclonal Antibody (H127C), eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Biotin (Cat. No. 13-7385-82)
• eFluor 660 (Cat. No. 50-7385-82)
• Custom conjugation available upon request

Product Specific Information

The monoclonal antibody H127C recognizes human IL-38, a member of the Interleukin-1 family (IL-1F) that shares homology with IL-1R antagonist (IL-1Ra) and IL-36R antagonist (IL-36Ra).
IL-38 is mainly expressed in the skin and proliferating B cells of the tonsil, with lower expression in the heart and placenta. It acts as an anti-inflammatory cytokine that inhibits IL-36 by binding to IL-36R and suppresses production of IL-17 and IL-22.
Gene polymorphisms of IL-38 are associated with psoriatic arthritis, ankylosing spondylitis, and heart disease.

Applications Reported: Flow cytometry, immunoprecipitation, western blotting, ELISA, and immunohistochemistry (formalin-fixed paraffin-embedded tissue).
Applications Tested: Tested on FFPE human tissue using low pH antigen retrieval; recommended ≤0.1 µg/mL with titration for optimal performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Cytokine IL-1 initiates multiple immune responses via activation of NF-κB and AP-1 transcription factors.
IL-1F10 (Interleukin-1 family member 10), also known as FIL1T, IL1HY2, or FKSG75, is highly expressed in skin, spleen, and proliferating B-cells of the tonsil. It binds soluble IL-1 receptor type 1 and is one of nine IL-1 family members clustered on chromosome 2.
IL-1F10 and IL-1RN polymorphisms are implicated in susceptibility to rheumatoid arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.