Thermo Fisher Scientific mGluR5 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

This antibody is predicted to react with bovine, canine, amphibian, mouse, rabbit, and rat based on sequence homology.

Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is brain tissue.

Target Information

GRK1 is a member of the guanine nucleotide protein-coupled (G protein) receptor kinase subfamily of Serine/Threonine kinase family.
GRK1 phosphorylates rhodopsin and initiates its deactivation. G protein-mediated phosphorylation promotes the binding of arrestin, which removes the receptor from the G-protein and terminates the receptor signal chain.
GRK1 is farnesylated to promote membrane association. Studies have shown that GRK1 can be inhibited by recoverin, a calcium-binding protein.
Diseases associated with GRK1 dysfunction include Oguchi Disease-2 and Hereditary Night Blindness.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.