Thermo Fisher Scientific MCM2 Polyclonal Antibody
Rabbit polyclonal antibody targeting human MCM2 protein N-terminus. Suitable for WB and IHC applications. Predicted cross-reactivity with multiple species. Ideal for studying DNA replication and cell cycle regulation.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to N-terminus of human MCM2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2142125 |
Product Specific Information
This antibody is predicted to react with bovine, canine, porcine, and rabbit based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is breast carcinoma tissue.
Target Information
MCM2 (Mini chromosome maintenance protein 2) regulates DNA replication and is essential for the onset of DNA synthesis and cell division. Also known as CDCL1, mitotin, and BM28, MCM2 is a nuclear protein mapped to chromosome 3q21 and is considered a potential oncogene candidate affected by chromosomal breaks in acute myeloid leukemia (AML).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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