Thermo Fisher Scientific Calnexin Monoclonal Antibody (AF18)
Calnexin 단백질을 인식하는 AF18 클론의 Mouse Monoclonal Antibody로, Human 및 Mouse 시료에 반응합니다. Western blot, ICC/IF, IP 등 다양한 응용에 적합하며, ER 특이적 염색 패턴을 제공합니다. 고순도 Protein A 정제, 1 mg/mL 농도, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:100–1:1,000 | 30 publications |
| Immunohistochemistry (IHC) | – | 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20 | – |
| Immunohistochemistry (Frozen) (IHC (F)) | – | 1 publication |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 | 33 publications |
| Immunoprecipitation (IP) | 5 µg | 3 publications |
| in situ PLA (PLA) | – | 1 publication |
| Immunomicroscopy (IM) | – | 1 publication |
| RNA Immunoprecipitation (RIP) | Assay-dependent | – |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Dog, Hamster, Human, Mouse, Non-human primate, Rat, Yeast |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AF18 |
| Immunogen | Focus human hepatoma cell lysate (Calnexin, UniProt ID: P27824-1, Range: 1–592) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.05% Sodium Azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2069043 |
Additional Formats:
- Alexa Fluor 488 (MA3-027-A488)
- Alexa Fluor 555 (MA3-027-A555)
- Alexa Fluor 647 (MA3-027-A647)
- Request custom conjugation
Product Specific Information
MA3-027 detects calnexin from human and mouse tissues.
It has been successfully used in Western blot, immunocytochemistry, immunofluorescence, and immunoprecipitation protocols.
By Western blot, this antibody detects calnexin in human liver, skin, adrenal gland, heart, colon, testes, and ovary extracts.
Not recommended for human kidney or mouse liver lysates in Western blot applications.
The predicted molecular weight is ~67 kDa; however, it may appear as ~90 kDa on SDS-PAGE due to C-terminal negative charges affecting SDS binding and mobility.
Immunocytochemical staining of calnexin in Huh-7 cells with MA3-027 yields a pattern consistent with endoplasmic reticulum localization.
The MA3-027 antigen is Focus human hepatoma cell lysate.
Target Information
Calnexin (also known as IP90, p88, p90) is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER).
It functions as a molecular chaperone involved in protein folding and assembly.
Calnexin associates with MHC class I heavy chains, partial T cell receptor complexes, and B cell membrane immunoglobulin, but not with completed receptor complexes.
It retains improperly folded proteins in the ER.
Soluble ER resident proteins often contain the KDEL sequence for retention, while integral membrane proteins like calnexin use positively charged cytosolic residues for ER localization.
Calnexin structure includes:
- ER luminal domain (461 amino acids)
- Transmembrane segment (22 amino acids)
- Cytoplasmic tail (89 amino acids)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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