Thermo Fisher Scientific Calnexin Monoclonal Antibody (AF18)

Applications and Tested Dilutions

Product Specifications

Additional Formats:

• Alexa Fluor 488 (MA3-027-A488)
• Alexa Fluor 555 (MA3-027-A555)
• Alexa Fluor 647 (MA3-027-A647)
• Request custom conjugation

Product Specific Information

MA3-027 detects calnexin from human and mouse tissues.
It has been successfully used in Western blot, immunocytochemistry, immunofluorescence, and immunoprecipitation protocols.
By Western blot, this antibody detects calnexin in human liver, skin, adrenal gland, heart, colon, testes, and ovary extracts.
Not recommended for human kidney or mouse liver lysates in Western blot applications.

The predicted molecular weight is ~67 kDa; however, it may appear as ~90 kDa on SDS-PAGE due to C-terminal negative charges affecting SDS binding and mobility.
Immunocytochemical staining of calnexin in Huh-7 cells with MA3-027 yields a pattern consistent with endoplasmic reticulum localization.
The MA3-027 antigen is Focus human hepatoma cell lysate.

Target Information

Calnexin (also known as IP90, p88, p90) is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER).
It functions as a molecular chaperone involved in protein folding and assembly.
Calnexin associates with MHC class I heavy chains, partial T cell receptor complexes, and B cell membrane immunoglobulin, but not with completed receptor complexes.
It retains improperly folded proteins in the ER.

Soluble ER resident proteins often contain the KDEL sequence for retention, while integral membrane proteins like calnexin use positively charged cytosolic residues for ER localization.
Calnexin structure includes:

• ER luminal domain (461 amino acids)
• Transmembrane segment (22 amino acids)
• Cytoplasmic tail (89 amino acids)

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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