Thermo Fisher Scientific Calnexin Monoclonal Antibody (AF18), Alexa Fluor 647
Human 및 Mouse 시료에서 Calnexin 단백질을 검출하는 Alexa Fluor 647 결합 단일클론 항체. ICC/IF 및 Flow Cytometry에 적합하며, 높은 특이성과 안정적인 형광 신호 제공. Protein A 정제, 4°C 보관, 연구용 전용.
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Applications
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1–10 µg/mL
Flow Cytometry (Flow)
- Tested Dilution: Not specified
- Publications: 1 available
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AF18 |
| Immunogen | Focus human hepatoma cell lysate |
| Conjugate | Alexa Fluor™ 647 |
| Excitation / Emission Max | 650 / 671 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2662811 |
Additional Formats
Product Specific Information
MA3027A647 detects calnexin from human and mouse tissues.
The MA3027A647 antigen is Focus human hepatoma cell lysate.
Target Information
Calnexin (also known as IP90, p88, and p90) is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER). It acts as a molecular chaperone, assisting in the proper folding and assembly of polypeptides into multisubunit proteins.
Calnexin associates with MHC class I heavy chains and partial complexes of the T cell receptor and B cell membrane immunoglobulin but not with completed receptor complexes.
It retains incompletely or improperly folded proteins within the ER.
Soluble ER resident proteins often contain the KDEL sequence for retention, while integral membrane ER proteins like calnexin lack KDEL but have positively charged cytosolic residues ensuring ER localization.
Calnexin consists of a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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