Thermo Fisher Scientific mtHSP70 Monoclonal Antibody (JG1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 95 publications 95 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1 µg/mL

-

Immunocytochemistry (ICC/IF)

1:50

View 32 publications 32 publications

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

in situ PLA (PLA)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 8 publications 8 publications

SDS-PAGE (SDS-PAGE)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human, Mouse, Non-human primate

Published species

Dog, Guinea pig, Hamster, Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG3

Class

Monoclonal

Type

Antibody

Clone

JG1

Immunogen

Synthetic peptide corresponding to residues G(615) S G S S G T G E Q K E D Q K E E K Q(633) of mouse mtHSP70. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:mtHSP70,uniProtId:Q61696-1,ncbiNodeId:10090,antigenRange:615-633,antigenLength:641,antigenImageFileName:MA3-028_mtHSP70_Q61696-1_House_mouse.svg,antigenImageFileNamePDP:MA3-028_mtHSP70_Q61696-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.07 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325474

Product Specific Information

MA3-028 detects mitochondrial heat shock protein 70 kDa (mtHSP70) from human, non-human primate, canine and mouse tissues.

MA3-028 has been successfully used in Western blot, immunocytochemical, immunofluorescence, immunohistochemical (paraffin), and immunoprecipitation procedures. By Western blot, this antibody detects a single ~75 kDa band representing mtHSP70 from U2OS cell homogenate. Immunocytochemical staining of mtHSP70 in DAP.3 cells with MA3-028 results in a worm-like staining pattern, consistent with mitochondrial localization.

Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.

Target Information

The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a 70 kDa protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein which is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa constitutively expressed protein which is involved in uncoating clathrin-coated vesicles; GRP78, or BiP, is a glucose-regulated 78 kDa protein localized to the endoplasmic reticulum; and mitochondrial HSP70 (mtHSP70, GRP75 or mortalin) a 75 kDa protein that is found within the mitochondria. mtHSP70 is a mitochondrial resident protein that is involved in protein translocation into the mitochondria. Preproteins cross the mitochondrial membranes in an extended conformation. This requires unfolding of preproteins before entering translocation pores in the mitochondrial outer membrane. Preprotein unfolding is thought to be mediated by mtHSP70 and other inner membrane proteins of the mitochondria.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.